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dc.contributor.advisorYurnaliza
dc.contributor.authorSarimunggu, Listra
dc.date.accessioned2019-03-11T02:52:54Z
dc.date.available2019-03-11T02:52:54Z
dc.date.issued2019
dc.identifier.otherAkhmad Danil
dc.identifier.urihttp://repositori.usu.ac.id/handle/123456789/12275
dc.description140805062en_US
dc.description.abstractSearch to effort biological agent to control basal stem rot disease in oil palm caused by Ganoderma boninense is still being done. The aim of this research was to obtain diazotrophic bacteria from humus of oil palm that have antagonistic activity against Ganoderma boninense, to evaluate their antagonistic mechanism and plant growth promoting activity such as nitrogen fixation, phosphate solubilization and indole acetic acid production. Diazotrophic bacteria were isolated from humus of oil palm plantation from 3 locations i.e. Universitas Sumatera Utara, community’s plantation in Bingkat village, PT. Perkebunan Nusantara IV Adolina Dusun I Tanjung Putus. Antagonistic activity was evaluated by dual culture method. Selected bacterial isolates were characterized and analyzed for their antifungal, chitinolytic and glucanolytic activity, indole acetic acid production, nitrogen fixation and phosphate solubilization. The results showed that humus of community’s plantation in Bingkat village had the highest population of diazotrophic bacteria. Sixteen of 90 selected diazotrophic bacteria were able to inhibit Ganoderma boninense with the highest inhibition percentage. Among the bacterial isolates, TU 9 was the highest inhibition percentage (55.5%). Fourteen isolates were able to produced antifungal compounds, seven isolates produced glucanase and no isolates produced chitinase. Two diazotrophic bacteria with the highest inhibitory activity, i.e. isolate TU 9 and TU 17 with inhibitory activity 55.5% and 44.4% consecutively. The isolates were able to produce indole acetic acid, perform nitrogen fixation and solubilize phosphate.en_US
dc.description.abstractPencarian agen pengendali hayati penyakit busuk pangkal batang pada kelapa sawit yang disebabkan oleh Ganoderma boninense masih terus dilakukan. Tujuan penelitian ini adalah mendapatan bakteri diazotrof dari humus kelapa sawit yang berpotensi menghambat Ganoderma boninense, mengetahui mekanisme penghambatan bakteri dan mendukung pertumbuhan tanaman seperti menambat nitrogen, melarutkan fosfat dan menghasilkan asam indol asetat. Bakteri diazotrof diisolasi dari humus kelapa sawit Universitas Sumatera Utara, kebun rakyat di desa bingkat, Serdang Bedagai dan PT. Perkebunan Nusantara IV Adolina Dusun I Tanjung Putus. Seleksi antagonis dilakukan dengan metode dual culture. Isolat terseleksi dikarakterisasi dan dianalisis kemampuan antijamur, aktivitas kitinolitik, glukanolitik, produksi asam indol asetat, menambat nitrogen dan melarutkan fosfat. Hasil isolasi diperoleh total koloni bakteri diazotrof terbanyak pada humus kebun rakyat di desa Bingkat. Sebanyak 16 dari 90 isolat bakteri diazotrof terpilih, mampu menghambat Ganoderma boninense pada uji antagonis dengan persentase penghambatan tertinggi. Diantara 16 isolat bakteri, TU 9 memiliki persentase hambatan tertinggi (55,5%). Sebanyak 14 isolat bakteri menghasilkan senyawa antijamur, 7 isolat bakteri menghasilkan glukanase dan tidak ada yang menghasilkan kitinase. Dua bakteri diazotrof dengan kemampuan antagonis tertinggi yaitu TU 9 dan TU 17 (55,5% dan 44,4%) mampu menghasilkan asam indol asetat, menambat nitrogen dan melarutkan fosfat.en_US
dc.language.isoiden_US
dc.publisherUniversitas Sumatera Utaraen_US
dc.subjectAntagonismen_US
dc.subjectAntifungalen_US
dc.subjectDiazotroph Bacteriaen_US
dc.subjectGanoderma Boninenseen_US
dc.subjectOil Palmen_US
dc.titleSeleksi Bakteri Diazotrof Isolat Humus Kelapa Sawit (Elaeis guineensis Jacq.) sebagai Agen Pengendali Ganoderma boninenseen_US
dc.typeSkripsi Sarjanaen_US


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