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dc.contributor.advisorHanafiah, Diana Sofia
dc.contributor.advisorDamanik, Revandy.I.M
dc.contributor.authorLestami, Anggria
dc.date.accessioned2020-01-03T03:12:12Z
dc.date.available2020-01-03T03:12:12Z
dc.date.issued2019
dc.identifier.urihttp://repositori.usu.ac.id/handle/123456789/22088
dc.description94 Halamanen_US
dc.description.abstractAnggria Lestami. 2019. “Diversity and genetic identification of soybean mutant (Glycine max (L) Merril) resistant Athelia rolfsii Curzi based on SSR markers”, supervised by Diana Sofia Hanafiah and Revandy I. M. Damanik. The objective of this research was to identify the genetic and genetic diversity soybean mutant lines resistant of Athelia rolfsii Curzi based on SSR markers. The study was conducted at the Laboratory of Plant Biotechnology, Faculty of Agriculture, Universitas Sumatera Utara, Medan in January-May 2019. There were 13 soybean mutant lines, Anjasmoro varieties as elders and Kipas Putih as comparative resistant Athelia rolfsii Curzi were analyzed descriptively analyzed by observing the pattern of DNA bands using 5 primers SSR Satt009, Satt114, Satt147, Satt191, Satt197. The results showed that the primers used were specific and polymorphic with PIC values ranging from 0.54 to 0.83 (included in the informative class). Based on the analysis of molecular variants (AMOVA), the diversity of soybean mutant lines 44% comes from the diversity between individuals in the population. Based on UPGMA phylogenetic analysis, there were 2 mutant lines which were identified as having Athelia rolfsii Curzi resistant character according to comparative varieties on the genetic similarity of 0.92, namely M100-A25 (3/7) and M200-A17 (13/6).en_US
dc.description.abstractAnggria Lestami. 2019. “Keragaman dan identifikasi genetik galur mutan kedelai (Glycine max (L) Merril) resisten Athelia rolfsii Curzi berdasarkan marka SSR”, dibimbing oleh Diana Sofia Hanafiah dan Revandy I. M. Damanik. Penelitian ini bertujuan untuk mengidentifikasi genetik dan keragaman genetik galur mutan kedelai resisten Athelia rolfsii Curzi berdasarkan marka SSR. Penelitian dilakukan di Laboratorium Bioteknologi Tanaman Fakultas Pertanian Universitas Sumatera Utara, Medan pada bulan Januari - Mei 2019. Sebanyak 13 galur mutan kedelai, varietas Anjasmoro sebagai tetua dan Kipas Putih sebagai pembanding resisten Athelia rolfsii Curzi dianalisis secara deskriptif dengan mengamati pola pita DNA menggunakan 5 primer SSR Satt009, Satt114, Satt147, Satt191, Satt197. Hasil penelitian menunjukkan bahwa primer yang digunakan spesifik dan polimorfik dengan nilai PIC berkisar 0,54-0,83 (termasuk dalam kelas informatif). Berdasarkan hasil analisis varian molekuler (AMOVA), keragaman galur mutan kedelai 44% berasal dari keragaman antar individu dalam populasi. Berdasarkan analisis filogenetik UPGMA terdapat 2 galur mutan yang teridentifikasi memiliki karakter resisten Athelia rolfsii Curzi sesuai dengan varietas pembanding pada kesamaan genetik 0,92 yaitu M100-A25(3/7) dan M200-A17(13/6).en_US
dc.language.isoiden_US
dc.publisherUniversitas Sumatera Utaraen_US
dc.subjectGalur Mutanen_US
dc.subjectResistenen_US
dc.subjectAthelia Rolfsiien_US
dc.subjectSSRen_US
dc.titleKeragaman dan Identifikasi Genetik Galur Mutan Kedelai (Glycine max (L.) Merril) Resisten Athelia rolfsii Curzi Berdasarkan Marka SSRen_US
dc.typeTesis Magisteren_US
dc.identifier.nimnipnik177001016


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