| dc.description.abstract | Background: The poguntano plant from the Linderniaceae family has
pharmacological activity as a diuretic, anticancer, antipyretic, hepatoprotective,
cardioprotective, anti-inflammatory, analgesic and anthelmintic. The contents of
poguntano herbs include flavonoids, glycosides, saponins, tannins and steroids.
Apigenin and quercetin are flavonoid derivatives in the flavone and flavonol
subclasses. The UHPLC method was used to examine apigenin levels in
poguntano herb extract.
Objective: The purpose of this research was to determine the levels of apigenin
and quercetin from the hydroalcoholic extract of the herb poguntano (Picria felterrae
Lour.) using the UHPLC method.
Method: The research was carried out by making simplicia, characterization of
simplicia, making hydroalcoholic extract of Poguntano herb using maceration
method, qualitative testing and determination of apigenin and quercetin levels
using UHPLC, using the mobile phase aquabides:acetonitrile:methanol
(50:30:20) with a flow rate of 0.5 mL/minute, temperature 35ºC, wavelength 350
nm, injection volume 10μL, and C18 (octadecyl) column. The method validation
test parameters carried out are linearity, accuracy, precision, limit of detection
(LOD), and limit of quantitation (LOQ).
Results: The results of the characterization of simplicia water content, water
soluble essence content, ethanol soluble essence content, total ash content,
insoluble ash content of simplicia acid were respectively 7.98%; 23.55%;
19.31%; 4.19%; 0.71% and water content, total ash content, extract acid insoluble
ash content respectively 9.28%; 2.32%; 0.43%.
The results of identification using UHPLC of Poguntano herb extract showed that
there were apigenin and quercetin compounds at a retention time of 5.562 minutes
and 4.017 minutes with levels of apigenin 0.0493 mg/g extract and quercetin
0.5060 mg/g extract. Validation test results obtained % recovery of apigenin
97.81% quercetin 102.95%; RSD apigenin 0.5537% quercetin 1.0668%; LOD
apigenin 0.0012 mg/g extract, quercetin 0.0004 mg/g extract; and LOQ apigenin
0.0038 mg/g extract, quercetin 0.0011 mg/g extract.
Conclusion: The results of this study showed that the levels of apigenin and
quercetin compounds in the hydroalcoholic extract of poguntano herb obtained
were 0.0493 mg/g ekstract dan 0.5060 mg/g extract. The UHPLC method used
to examine apigenin and quercetin levels has met the validation requirements. | en_US |
