Isolasi dan Identifikasi Golongan Senyawa Flavonoida dari Daun Mahang Biru (Macaranga Heynei I. M. Johnst)

Abstract

Isolation and identification of the flavonoid compound from mahang biru (Macaranga heynei I. M. Johnst.) leaves had been done by maceration extraction technique with methanol solvent. Methanol solid extract was re extracted with ethyl acetate repeatedly until negative to FeCl3 5%. Ethyl acetate extract was dissolved with methanol and partitioned with n hexane until it was clear. The concentrated methanol extract was then analyzed by thin layer chromatography and separated by column chromatography with chloroform : ethyl acetate (90:10; 80:20; 70:30; 60:40; 50:50; 40:60; 30:70; 20:80; 10:90) v/v eluent, respectively. The compund obtained was purified by preparative thin layer chromatography with chloroform : ethyl acetate (30:70) v/v. eluent and yellow amorphous solid was obtained as much as 5 mg with Rf 0,56 using chloroform : ethyl acetate (30:70) v/v. The UV Visible spectrum show a wavelength (λmax) 317 nm and 270 nm. The FT IR spectrum shows the presence of O H, C H sp3, C=O ketone, C=C aromatic, C O C, C H sp2 respectively according to wave 3425,58 cm 1; 2922,16 cm 1; 1726,29 cm 1; 1598,99 cm 1; 1122,57; 742,59 cm 1. The 1H NMR shows the presence of protons H 3; H 6; H 8; H 2’; H 3’; H 5’; H 6’. Based on the data obtained, the isolated compound is a flavonoid compound in the flavone group.