Isolasi dan Identifikasi Golongan Senyawa Flavonoida dari Kulit Batang Mahang Damar (Macaranga Triloba (Thunb.) Mull.Arg.)

Abstract

Isolation and identification of flavonoid compounds from stem bark of mahang damar (Macaranga triloba (Thunb.) Mull.Arg.) had been carried out. Isolation was carried out through maceration extraction using methanol and was concentrated. The concentrated methanol ectract was re-extracted using ethyl acetate. The ethyl acetate ectract was concentrated and the dissolved using methanol, then the partition process was carried out using n-hexane until the n-hexane layer was clear. Thin layer chromatography analysis was performed on the concentrated methanol ectract and then separated using column chromatography with sillica gel as stationary phase and mobile phase of eluent cloroform : ethyl acetate (90:10; 80:20; 70:30; 60:40; 50:50; 40:60; 30:70; 20:80; 10:90, v/v). The isolated compound from fraction 206 -280 was purified using preparative thin layer chromatography. A yellowish brown amorphous solid of 5,2 mg was obtain with a Rf = 0,71. Base of Ultraviolet visible (UV-Vis) spectrum with methanol solvent, it show that there are teo spectrum peak with wavelengeths (λmax) = 304 nm and 270 nm. The fourier transformed infrared (FT-IR) spectrum show the presence of O-H, C-H sp3 stretch, C=O, C=C aromatic, C-O-C and C-H sp2 bending out-of-plane groups respectively at the wave number 3427,51; 2920,23; 1639,49; 1562,34; 1234,44 and 804,31 cm-1. Proton Nuclear Magnetic Resonance Spectrum (1H-NMR) show the presence of H-2’; H-6’; H-3’; H-5’; H-6; H-8; H-2; H-3ax and H-3eq protons. Spectroscopic data show that isolate compound is flavanone group.