Isolasi dan Identifikasi Golongan Senyawa Flavonoida dari Kulit Batang Landoyung (Litsea Cubeba (Lour.) Pers.)

Abstract

Isolation and identification of flavonoid compound groups from landoyung bark (Litsea cubeba (Lour.) Pers.) had been carried out through maceration extraction stages with methanol solvent. The methanol extract was concentrated and then dissolved with distilled water, then partitioned with ethyl acetate, then the ethyl acetate extract was evaporated and concentrated. The concentrated ethyl acetate extract was dissolved with methanol and partition extracted with n-hexane. The methanol layer was evaporated until all the methanol had evaporated. The concentrated methanol extract was analyzed by TLC, then separated by column chromatography with silica gel as the stationary phase and chloroform: ethyl acetate (90:10, 80:20, 70:30, 60:40, 50:50, 40:60, 30:70, 20:80, 10:90) v/v as the mobile phase. The fourth fraction was selected for purification by column chromatography with silica gel as the stationary phase and chloroform:ethyl acetate (20:80) v/v as the mobile phase. The pure compound obtained was a yellow solid as much as 6 mg with Rf = 0.55 using chloroform:ethyl acetate (20:80) v/v as the eluent. Based on UV-Visible Spectroscopy Analysis with methanol solvent, it showed two absorptions with wavelengths (λmax) of 305 and 265 nm. Infrared Spectroscopy (FT-IR) showed the presence of O-H, C-H sp3 stretching, C=O ketone, C=C aromatic, C-H sp3 bending, C-O-C and C-H sp2 groups. Proton Nuclear Magnetic Resonance Spectroscopy (1H-NMR) showed the presence of protons H-3, H-6, H-8, H-2’, H-3’, H-5’, H-6’ and OCH3. Based on analysis data, the isolated compound from the landoyung bark extract is a flavonoid compound of the flavone group.