Isolasi dan Identifikasi Golongan Senyawa Flavonoida dari Kulit Batang PohonTrembesi (Samanea Saman (Jacq.) Merr)

Abstract

Isolation and identification of flavonoid compound groups from the bark of rain tree (samanea saman (jacq.) Merr) had been carried out through the stages of maceration extraction using methanol solvent. Extraction was carried out again with ethyl acetate, partitioned with n-hexane and obtained concentrated methanol extract. The concentrated methanol extract was then analyzed by TLC, then separated by silica gel column chromatography as the stationary phase and a mixture of chloroform: ethyl acetate 90:10; 80:20: 70:30; 60:40; 50:50; 40:60; 30:70; 20:80; 10:90 (v/v) as the mobile phase. The pure compound obtained was in the form of a yellow amorphous solid as much as 5,7 mg with an Rf value = 0.58 using chloroform: ethyl acetate 40:60 (v/v) eluent. Based on the analysis of UV Vis spectrophotometer with acetone solvent, it shows two absorption peaks with wavelengths (λmax) of 312 nm and 263 nm. The FT-IR spectrum shows the presence of O-H, C-H sp3, C=O ketone, C=C aromatic, C-H sp3, C-O-C, C-H sp2 groups, which are respectively at wave numbers 3446.79; 2922.16; 1726.29; 1600.92; 1382.96; 1274.95; 788.89 cm-1. Proton Nuclear Magnetic Resonance Spectroscopy (1H-NMR) shows the presence of protons H-5, H-6, H-2’, H-3’,H-5’, H-6’, and OCH3 protons. Based on the analysis data, the isolated compound is suspected to be a flavonoid compound of the flavonol group.