Uji Efektivitas Anti Helmintik dari Ekstrak Etanol Biji Cucurbita Moschata Menggunakan Tubifex Assay

Abstract

Background. Upsurge in soil-transmitted helminth infections pose a global threat to the world’s population. The escalation of soil-transmitted helminth are directly propotional with widely inappropriate usage of anthelmintics which arouse suspicions of anthelmintics resistancy. Prolonged anthelmintics resistance leads to an increased side effects such as malnutrition and stunting in the long run.One of the alternative used to replace conventional antihelminth drugs is yellow pumpkin seeds. Yellow pumpkin seeds are known for it’s property as a traditional anthelmintic in India, China, and South East Asia because it contains secondary metabolite such as triterpenoids, flavonoids, alkaloids, saponins and tannins proven to kill helminths. Objective. This study aims to determine the efficacy of yellow pumpkin seed ethanol extract as an alternative anthelmintic on Tubifex sp. Method. This is an in vitro experimental study. This study consisted 5 groups with 30 Tubifex sp. worms each, i.e., normal group, control group, E55 group (55% yellow pumpkin ethanol extract), E62 group (62.5% yellow pumpkin ethanol extract), E70 group (70% yellow pumpkin ethanol extract) and the lethal time of all worms in each group are measured. Data analysis used were ANOVA, T-independent, and Pearson Correlation test. Result. Yellow pumpkin seed ethanol extract at 55%, 62.5%, and 70% caused significant death time compared to albendazole control group. Increase in extract concentration did not have a remarkable impact on worms death. Conclusion. Yellow pumpkin seed ethanol extract presents antihelmintic property which caused significant helminth’s death in vitro.