Uji Daya Antibakteri Ekstrak Etanol Daun Teh Hijau (Camellia sinensis) pada Pertumbuhan Bakteri Escherichia coli ATCC 25922

Abstract

Background. The increasing prevalence of antibiotic resistance has drawn attention to the treatment of urinary tract infections (UTIs) brought on by Escherichia coli pathogens. The rise in cases of antibiotic resistance offers a chance for studying bioactive substances derived from natural sources, including green tea plants, as a substitute. Objective. To assess the antibacterial effectiveness of green tea leaf extract (Camellia sinensis) in inhibiting the growth of Escherichia coli. Method. This research utilized a true experimental design using a posttest-only control group methodology. The treatment group was administered ethanol extract of green tea leaves at doses of 6.25%, 12.5%, 25%, 50%, and 100%. The control group comprised a negative control with purified water and a positive control with Ciprofloxacin. The antibacterial activity test was performed via the disk diffusion method, wherein the inhibition zone was measured with a caliper. Results. The research results indicate that the ethanol extract of green tea leaves at doses of 6.25%, 12.5%, 25%, 50%, and 100% demonstrated differing inhibitory effects against E. coli, as assessed by the Kruskal-Wallis test, with a p value<0.05 for each group tested. The optimal efficacy of ethanol extract from green tea leaves was attained at the greatest concentration (100%). Conclusion. A zone of inhibition against E. coli was produced by the ethanol extract of green tea leaves, suggesting its potential as a natural or herbal antibacterial agent.