| dc.description.abstract | Sterilization of explants is an important stage in the garlic propagation
process because tubers as explant material often carry pathogens from the field.
This research aims to determine the most effective sterilization method for local
cultivars of North Sumatra garlic (Allium sativum L.). This research was carried
out at the Tissue Culture Laboratory, Faculty of Agriculture, University of North
Sumatra from May to August 2024, using a Non-Factorial Randomized Group
Design (RGD) with several treatments, namely S1 (Nordox 6 g/L 1 hour), S2
(Nordox 6 g /L 1 hour + Nordox 3 g/L 30 minutes), S3 (Nordox 6 g/L 1 hour +
Clorox 20% 30 minutes), S4 (Dithane 7.5 g/L 1 hour + Dithane 3.75 g/L 30
minutes), S5 (Dithane 7.5 g/L 1 hour + Clorox 20% 30 minutes). The results
showed that certain sterilization methods had a real influence on several
parameters, such as the percentage of explants that were not contaminated at 2-5
WAP, the percentage of live explants at 3-5 WAP, and the percentage of explants
free of fungal contamination at 3-5 WAP, but had no effect. significant to the
percentage of explants that were not contaminated with bacteria at 1-5 WAP.
Apart from that, the sterilization method also affects the number of leaves at 1-3
WAP. Treatment S3 and treatment S5 are the best treatments for sterilizing
explants but their growth is thought to be suppressed due to the influence of
Clorox, the dose of which is too high. The S3 method provides the best results on
the percentage of live explants compared to other methods. In contrast, methods
S1 (Nordox 6 g/L), S2 (Nordox 6 g/L + Nordox 3 g/L), and S4 (Dithane 7.5 g/L +
Dithane 3.75 g/L) produced better explant growth. normal and produces roots, but
the level of explant sterilization is very low and its viability is also low. | en_US |
