Uji Aktivitas Hepatoprotektif Ekstrak Etanol Daun Mobe (Artocarpus lacucha Buch-Ham.) pada Tikus yang Diinduksi Parasetamol

Abstract

Mobe (Artocarpus lacucha Busch - Ham.) contain secondary metabolite compounds such as flavonoids, tannins, saponins and glycosides. Mobe have anti-diabetic, antibacterial and anti-inflammatory activities. This study aims to test the hepatoprotective activity of ethanol extract of mobe leaves in paracetamol-induced rats. Ethanol extract of mobe leaves (EEDM) was extracted by maceration method using ethanol solvent. The extract was tested for sample characterization and identification of secondary metabolites was carried out using KLT densitometry. Hepatoprotective test of EEDM was conducted on male white rats induced with Paracetamol 2000 mg/KgBB for 9 days and biomarker measurements were carried out on day 10. Tests conducted to measure markers of liver damage ALT, AST, Total Bilirubin and ALP as well as measurements of SOD, Caspase 3, TNF α, IL 6, LDH and histopathological observations. Data analysis using the SPSS 22 program determined the normality of the data based on the statistical analysis used. The results showed that EEDM positively contained secondary metabolite compounds of flavonoids, glycosides, saponins, tannins, and steroids/triterpenoids. Results of sample characterisation moisture content 5.15%; water soluble juice content 11.10%; ethanol soluble juice content 3.93%; total ash content 2.77%; acid insoluble ash content 0.34%. The results showed that paracetamol administration at a dose of 2000 mg/KgBB can increase the risk of hepatotoxicity with an increase in ALT, AST, Total Bilirubin and ALP values. Judging from the test results, the higher the dose of EEDM given can reduce these parameters and there are significant differences between test groups (p<0.05). Histopathological observation of the liver can be seen that the higher the dose of EEDM, the darker the colour of the liver. A pale liver indicates liver damage. EEDM administration can help reduce the value of caspase-3, IL-6, LDH and TNF-α and can help increase the level of SOD as hepatotoxic parameters. This study suggests that EEDM has hepatoprotective activity against paracetamol-induced liver damage in rats.