Gambaran Histologi Pankreas Tikus (Rattus norvegicus) yang Diinduksi 7,12-Dimetilbenz-[a] Antrasena (DMBA) Dengan Pemberian Ekstrak Etanol Kulit Durian (Durio zibethinus Murr.)

Abstract

Durian (Durio zibethinus Murr.) contains secondary metabolite compound, flavonoids metabolite compound has a role as an antioxidant. Flavonoid compounds are able to capture and neutralize free radicals so they can repair cell damage. The research aims to determine the effect of administering durian peel extract (Durio zibethinus Murr) on the histology of rat pancreas (Rattus norvegicus) induced by 7,12 Dimethylbenz-[A] Anthracene (DMBA). The research used a Completely Randomized Design (CRD) with six treatments, namely: negative control (only given food ad libitum); positive control (induced by DMBA 10 mg/kgBB); DMBA induction + methotrexate 9mg/kgBB; and DMBA induction + durian peel extract 150, 300 and 450mg/kgBB. Histology of the pancreatic organ was made using the paraffin method and Hematoxylin Eosin (HE) staining. The research results showed that durian skin extract at a dose of 150 mg/kgBW had the potential to improve the diameter of the islets of Langerhans, necrosis, vacuolization and congestion.