Perbedaan Pengaruh Pengolesan Pasta Nanohidroksiapatit 12% dari Cangkang Telur Bebek (Anas platyrhyncos) dengan Casein Phosphopeptide-Amorphous Calcium Phosphate terhadap Kekerasan Permukaan Email (In Vitro)

Abstract

Caries is a chronic disease that occurs due to a demineralization process that can reduce the surface hardness of the enamel so that a remineralization material is needed, one of which is CPP ACP and the use of duck eggshells from natural materials as remineralization which is now widely researched. The purpose of the study was to determine the difference in the effect of applying 12% nanohydroxyapatite paste from duck eggshells and CPP-ACP as a remineralization material on enamel surface hardness. This study used 24 premolar teeth that had been extracted, embedded in plaster stone with the buccal surface facing up and divided into four groups, namely group I demineralized and given 12% duck eggshell nanohydroxyapatite paste, group II demineralized and given CPP-ACP paste, group III only demineralized, and group IV without demineralization or remineralization. Demineralization and remineralization were performed for 30 minutes. All samples were then immersed in artificial saliva and stored at 37°C for 14 days. Enamel surface hardness was performed using a Micro Vickers Hardness Tester at 3 points on the buccal surface of the teeth after demineralization and remineralization. The results of the t-paired test showed an increase in enamel surface hardness after administration of the test material in each group (p=0.0001). From the results of the One Way ANOVA test, the four groups showed differences in the effect of treatment on enamel surface hardness (p=0.0001). And the results of Post Hoc LSD showed a significant difference between the 12% nanohydroxyapatite paste group from duck eggshell and CPP-ACP (p=0.0001). The results showed that there was a difference in the effect of 12% nanohydroxyapatite paste and CPP-ACP on enamel surface hardness.