Analisis Perbedaan Pola Kuman Pada Pemeriksaan Kultur Jaringan Dan Kultur Pus Pada Pasien Dengan Ulkus Kaki Diabetikum Di RSUP H. Adam Malik Medan

Abstract

Background: Diabetic foot ulcer (DFU) is one of the chronic complications of diabetes mellitus that can increase the risk of amputation and lead to high treatment costs if the infection is not properly managed. Identifying the etiology of the infection is crucial for determining effective treatment. However, in Indonesia, particularly at H. Adam Malik General Hospital in Medan, there has been no clear study focusing on identifying diabetic foot infection patterns based on tissue culture. The Infectious Diseases Society of America (IDSA) 2023 recommends using tissue culture techniques for bacterial identification, moving away from pus cultures. Objective: This study aims to analyze the differences in infection patterns obtained through pus culture and tissue culture in patients with diabetic foot ulcers. Methods: This prospective descriptive study involved 41 patients treated at H. Adam Malik General Hospital, Medan. Ulcer samples were collected using two methods: sterile swabs for pus culture and tissue samples for tissue culture. The culture results were analyzed using the Kruskal-Wallis statistical test to compare the differences in bacterial patterns. Results: Gram-negative bacteria dominated both culture methods, with Pseudomonas spp being the most common in pus cultures and Escherichia coli in tissue cultures. A significant difference was found between the two methods in bacterial identification (p < 0.05).Conclusion: There is a significant difference between tissue culture and pus culture in diabetic foot ulcers, particularly in the number and types of bacterial isolates, with a p-value of 0.002. This difference is not coincidental, supporting IDSA’s recommendation to prioritize tissue culture over swab culture. These findings are consistent with other studies in the field.