Penentuan Kadar Quercetin dari Ekstrak Menikah dan Sediaan Kombinasi Ekstrak Ikan Gabus dengan Metode Spektrofotometer

Abstract

Background: Quercetine is a flavonoid compound found in many vegetables and fruits. In addition to having very strong antioxidant activity, quercetine also has other biological activities such as antiviral, antibacterial, anti-inflammatory, and anticancer. Several studies have shown that quercetine has significant activity in inhibiting several cancer cells such as breast, prostate, colon and lung cancer. Objective: This study aims to quantitatively analyze the quercetine levels of meniran extract and a combination of temulawak extract, meniran extract and snakehead fish extract using a spectrophotometer method. Method: The samples tested in this study were meniran extract and a combination of temulawak extract, meniran extract and snakehead fish extract. The method used to determine quercetine levels was the spectrophotometric method. Results: The results of quercetine levels in the meniran extract samples and the combination preparation of temulawak extract, meniran extract and snakehead fish extract which were carried out with three measurements (triple) were 0.89% and 0.33% respectively. Conclusion: The UV-VIs Spectrophotometry method can be used to analyze quercetine levels because quercetine contains a conjugated aromatic system that shows strong absorption bands in the ultraviolet and visible light spectrum. Samples of meniran extract and a combination of temulawak extract, meniran extract and snakehead fish extract have quercetine levels of 0.89% and 0.33%, respectively.