Pengaruh Perbedaan Metode Ekstraksi Maserasi dan Microwave Assisted Extraction Terhadap Aktivitas Antioksidan Pada Ekstrak Etanol Daun Iler (Colues scutellarioides [L.] Benth)

Abstract

Background: Iler leaf (Coleus scutellarioides (L.) Benth) is one of the plants that is efficacious as a natural antioxidant. Traditionally, iler leaves are widely used in West Java in therapeutic uses such as overcoming postpartum, dermatitis, abdominal pain, cough and pain in muscles, bronchitis, asthma, angina, indigestion, and animal bites. Based on this, researchers are interested in testing the effect of extraction methods on the antioxidant activity of ethanol extracts of iler leaves using the DPPH (2,2-diphenyl-1-picrylhdrazil) method. Objective : This study aims to compare the antioxidant activity of ethanol extract of iler leaves obtained by maceration and microwave assisted extraction (MAE) methods. Methods : This study includes the preparation of simplisia powder determination of water content, determination of water soluble juice content, determination of ethanol soluble juice content, determination of total ash content, and determination of acid insoluble ash content, phytochemical screening, extraction by maceration method and microwave-assisted extraction (MAE) using 96% ethanol and then antioxidant activity test against DPPH (2,2-diphenyl-1- picrylhydrazil). Result : The results showed that the characterization results of iler leaf simplisia powder, moisture content 8,65%, water soluble juice content of 23,33%, ethanol soluble juice content of 21,02%, total ash content of 6,79%, acid insoluble ash content of 1,77%. The results of phytochemical screening of ethanol extracts of iler leaves maceration and MAE were positive for flavonoids, saponins, glycosides, triterpenoids. IC50 value of ethanol extract of iler leaves maceration 56,2968 µg/mL, IC50 of ethanol extract of iler leaves MAE 48,2665 µg/mL while quercetin IC50 value of 4,5583 µg/mL. Conclusions : The results showed that ethanol extract of iler leaves obtained by microwave-assisted extraction (MAE) had better antioxidant activity than ethanol extract of iler leaves obtained by maceration.