Isolasi Senyawa Flavonoid dan aktivitas Antioksidan Ekstrak Etil Asetat Biji Okra (Abelmoschus esculentus L.) sebagai sediaan Formulasi Face Toner.

Abstract

Okra fruit (Abelmoschus esculentus L.) from the Malvaceae family has seeds that are thought to have flavonoid secondary metabolite compounds and have antioxidant activity that can be utilized as a preparation for face toner formulations. This study aims to isolate and utilize okra seeds as antioxidants. Phytochemical screening test results were positive for flavonoids in various extracts and isolates except n-hexane extract. The results of ethyl acetate fractionation were separated by column chromatography and purified by preparative thin layer chromatography to obtain a yellow amorphous solid of 10 mg with an Rf price of 0.44: 0,44. Antioxidant activity was tested using DPPH (1,1 diphenyl-2-picrylhydrazyl) method with UV-vis spectrophotometer (maximum wavelength 517 nm). Sample concentration variations were 6.25; 12.5; 25; 50 and 100 µg/mL. The antioxidant activity of ethyl acetate extract of okra seed showed an IC50 value of 40.90 µg/mL, while for each face toner formulation: toner + BHT, toner + okra seed extract, trademark toner has antioxidant activity with IC50 values respectively: 85.67 µg/mL, 88.79 µg/mL, 105.69 µg/mL. UVVis spectrophotometer analysis with methanol solvent showed two absorption peaks with wavelengths (λmax) of 357 nm and 253 nm. Infrared spectrophotometer (FT-IR) showed the presence of O-H, C=O ketone, C=C aromatic, C-H and C-O-C groups. Proton core magnetic resonance spectrophotometer (¹H- NMR) showed the presence of protons H-6, H-8, H-2' & H-6', CH3, CH2 and O-CH3 Liquid chromatography-mass spectrometry (LC-MS) has confirmed the presence of 3-OH substituted flavonol flavonoid compounds. The 3-OH-substituted flavonol compound has the molecular formula C19H18O7 with a molecular weight of 361 g/mol. From the spectrum analysis, it is concluded that the isolated compound is a flavonoid compound with a 3-OHsubstituted flavonol group, namely 3-OH substituted flavonol