| dc.description.abstract | Houseflies (Musca domestica L.) are vector that carry disease-causing agents microorganisms from one environment to the others. The presence of houseflies at the hospital environment is likely to carry pathogenic bacteria like Staphylococcus sp., which is resistant to several antibiotics to surrounding area. This study aims to determine the sensitivity level of Staphylococcus sp. from houseflies collected at waste disposal sites of two hospital in Medan City to several antibiotics. Houseflies were collected from four points at two hospitals in Medan City, specifically from the waste disposal sites and the surrounding hospital areas. Isolation of bacteria from houseflies’ legs was carried out using Mannitol Salt Agar (MSA) media. Isolation was also carried out using MSA media with the addition of Amoxicillin at three concentrations: 25 µg/mL, 50 µg/mL dan 75 µg/mL. Bacterial colonies suspected to Staphylococcus species were characterized morphologically, by Gram characteristics and biochemical tests. Total of 10 Staphylococcus sp. isolates were selected for sensitivity testing to the antibiotic Penicillin 10 units, Gentamycin 10 µg, Erythromycin 15 µg, Oxacillin 30 µg and Tetracycline 30 µg using the Agar Diffusion (Kirby-Bauer) method. The total number of bacterial colonies/ten houseflies obtained from four points at two hospitals ranged from 6,7 x 105 CFU to 2,87 x 106 CFU, with the highest total of bacterial colonies were found at Rumah sakit A and its surrounding area. In the isolation media added with Amoxicillin, it was obtained that the number decreased as the concentration of Amoxicillin increased. The highest number of Amoxicillin-resistant bacterial colonies was obtained at Rumah Sakit A and its surrounding area. The result of the sensitivity testing of 10 Staphylococcus sp. isolates were resistant to Penicillin 10 units. Total of 3 isolates US02, US03, US04 were resistant to Gentamycin 10µg, Erythromycin 15 µg and Oxacillin 30 µg. The other isolates were intermediate or sensitive to antibiotics, except Penicillin 10 units. The results of this sensitivity testing showed an overview to the importance of proper hospital waste management so that the antibiotics-resistant bacteria will not spread to the surrounding area.
Keywords: Staphylococcus sp., houseflies (Musca domestica L.), Mannitol Salt Agar, antibiotic resistance | en_US |
