| dc.description.abstract | Candida albicans is a fungus that causes oral candidiasis or oral thrush. The
way to treat oral thrush is to use antifungals. Basil leaves (Ocinum basilicum L.) have
antifungal substances in the form of essential oils, flavonoids, alkaloids, tannins, and
phenols. The aim of the research was to determine the inhibition zone, minimum
inhibitory concentration (MIC) and minimum kill concentration (KBM) of basil leaf
extract against Candida albicans from oral thrush sufferers at concentrations of 12,5%,
25%, 37,5% and 50%. The method used was laboratory experimental with a post test
only control group design. Determination of the inhibition zone using the standard
Kirby Bauer blank disk diffusion method was measured using digital slide calipers,
while the KHM and KBM dilution methods were used to determine the level of
turbidity and clarity. Basil leaves were extracted using the maceration method, grouped
into 4 treatments and 4 replications. Analysis of inhibition zone diameter data using
the Kruskal Wallis test followed by the Mann Whitney test, then the number of colonies
using the Oneway Anova test followed by the Post Hoc Least Significance Difference
(LSD) test to determine significant differences in each concentration. The research
results showed that the average diameter of the inhibition zone at concentrations of
12,5%, 25%, 37,5% and 50% was 0 mm, 10,56 mm, 13,31 mm and 21,63 mm.
Minimum Inhibitory Concentration Value (MIC) at a concentration of 25%. Minimum
Kill Rate (KBM) at a concentration of 50%. The conclusion is that there is significant
potential for basil leaf extract on the growth of Candida albicans with a significance
value (p=0.000), the diameter of the strongest inhibitory zone with a concentration of
50% has an average diameter of 21,63 mm, the higher the concentration, the potency
of the extract will increase. | en_US |
