Uji Efektivitas Antibakteri Ekstrak Buah Kecombrang (Etlingeraelatior (Jack) R.M. S.m) Terhadap Pertumbuhan Enterococcus faecalis (In Vitro)

Abstract

Enterococcus facealis is a gram-positive facultative anaerobic bacteria in the form of a coccus. Enterococcus facealis has long been known to play a role in root canal infections and is resistant to medications during root canal treatment, causing root canal treatment failure. The prevalence of infection caused by Enterococcus facealis bacteria during root canal treatment is quite high ranging from 24%-77%. The part of kecombrang (Etlingera elatior) that has been tested for effectiveness against antibacterial properties, especially on E. faecalis bacteria, is the fruit. Kecombrang fruit can inhibit the growth of E. faecalis bacteria at various concentrations and has potential as an alternative irrigation material. This type of research includes laboratory experimental research with a post test only research design with controlled group design. The samples used were Enterococcus faecalis and 70% ethanol extract of kecombrang fruit (Etlingera elatior) with 8 test concentration groups and 2 control groups. The method used Kirby-Bauer disc diffusion to determine the value of the minimum inhibitory concentration (KHM) and streaking method from KHM testing to determine the value of the minimum kill concentration (KBM). Data analysis of the results of the study to determine the value of KHM and KBM using the One-Way ANOVA parametric test (p<0.05) and continued with the Post Hoc Test (LSD). The results showed that there was antibacterial activity of kecombrang fruit extract (E.elatior) against the growth of Enterococcus faecalis bacteria. Kecombrang fruit extract (E.elatior) is able to inhibit the growth of Enterococcus faecalis bacteria starting from a concentration of 35% with a mean inhibition zone diameter of 22.033 ± 0.2517 mm and a killing power of 35% to a concentration of 60% with the smallest killing power of 43.78% and a killing power of 98.85%. This study concluded that the concentration of 35% was determined as the KHM value. The KBM value among the test concentrations was at a concentration of 60%. The effective concentration of kecombrang (Etlingera elatior) flower extract that has inhibition and killing power so that it has the potential to become a root canal irrigation material is 60% concentration.