Uji Efektivitas Nanoherbal Daun Gagatan Harimau (Paraboea leuserensis B.L.Burtt.) terhadap Histologi Testis Tikus Wistar (Rattus norvegicus L.) yang Diinduksi Doksorubisin

Abstract

Doxorubicin is an antibiotic in the anthracycline group that is commonly used as a chemotherapeutic agent. However, doxorubicin has toxic effects on the testes. Testicular damage caused by doxorubicin is believed to be primarily due to oxidative stress. Gagatan harimau (Paraboea leuserensis B.L.Burtt) contains secondary metabolites such as flavonoids and alkaloids that act as antioxidants and promote the repair of organ histological structures, especially due to its quercetin content. This study aims to determine the effect of nanoherbal extract of gagatan harimau leaves on the morphology and histology of Wistar rat (Rattus norvegicus L.) testes induced by doxorubicin. The study consisted of four groups with six replications using a Completely Randomized Design (CRD), namely: (1) K- (negative control, no treatment); (2) K+ (doxorubicin-induced); (3) K125 (doxorubicin-induced + gagatan harimau nanoherbal 125 mg/kg BW); (4) K150 (doxorubicin-induced + gagatan harimau nanoherbal 150 mg/kg BW). The results showed that the administration of gagatan harimau nanoherbal at a dose of 150 mg/kg BW significantly increased testicular weight and improved testicular histology (p<0.05), including the diameter of seminiferous tubules, germinal epithelial thickness, number of Leydig cells, spermatogonia, spermatocytes, and spermatids.