Pengaruh Nanoherbal Daun Gagatan Harimau (Paraboea leuserensis B.L.Burtt.) Terhadap Histologi Retina Tikus Wistar (Rattus norvegicus L.) Jantan Yang Diinduksi Doksorubisin

Abstract

Doxorubicin is a chemotherapy drug from the anthracycline group that is effective in inhibiting DNA replication in cancer cells; however, its use may cause toxic effects on visual organs, including the retina. The tiger gagatan leaf contains various secondary metabolites such as flavonoids and terpenoids, which act as antioxidants and has the potential to protect cells and tissues from damage. This study aimed to determine the effect of nanoherbal tiger gagatan leaf administration on the histological structure of the retina in doxorubicin-induced rats (Rattus norvegicus L.). The research used a completely randomized design (CRD) with 4 groups and 6 replications. The groups in the study consisted of T0 (without treatment); T+ (doxorubicin 0,0019 mg/kg BW; T125 (doxorubicin 0,0019 mg/kg BW+nanoherbal tiger's claw leaf 125 mg/BW); and T150 (doxorubicin 0,0019 mg/kg BW+nanoherbal tiger's claw leaf 150 mg/kg BW). The eye organ preparations were made by using the paraffin method and Hematoxylin-Eosin (HE) staining. The results show that the administration of nanoherbal tiger gagatan leaf has no significant effect on eye weight (p>0,05), but significantly increases the thickness of the ganglion cell layer (GCL) at a dose of 150 mg/kg BW (p<0,05). There is no significant differences are observed in the IPL, INL, OPL, and ONL layers (p>0,05).