Determinasi Kadar Total Fenol, Flavonoid dan Antioksidan dari Ekstrak Etanol dan Ekstrak Kaya Polifenol Herba Pegagan (Centella asiatica (L.) Urb.)

Abstract

the acquisition of a level of active substance compounds, one of which is the variation of the extraction method used. Objective: This study aims to compare the levels of total phenols, flavonoids and antioxidant activity of Gotu Kola herb extracts produced through conventional maceration method and polyphenol-rich extraction method. Method: The research was conducted experimentally including sample preparation, making Gotu Kola herb extract, characterization of simplisia, phytochemical screening, determination of total phenol content using Folin-Ciocalteu reagent with gallic acid standard, determination of total flavonoids using AlCl3 reagent with quercetin standard. Determination of antioxidant activity using DPPH (1,1-diphenyl-2- picrylhydrazil) method with quercetin as a comparator, then measured using UV-Vis spectrophotometer. Results: The highest phenol content was obtained in the polyphenol-rich extract which was 109.2609 ± 0.2131 mg GAE/g sample and the lowest in the ethanol extract which was 68.5510 ± 0.8647 mg GAE/g sample. The highest flavonoid content in the polyphenol-rich extract was 88.6928 ± 1.0110 mg QE/g sample and the lowest in the ethanol extract was 56.4924 ± 1.3717 mg QE/g sample. The antioxidant activity of polyphenol-rich extract had the best IC50 value of 53.3779±0.0273 μg/ml and ethanol extract had IC50 of 63.8465±0.0273 μg/ml. Conclusion: The results showed that there were differences in the levels of total phenols, total flavonoids, and antioxidant activity of ethanol extracts and polyphenol-rich extracts of Gotu Kola herbs.