Uji Aktivitas Hemostasis dan Penyembuhan Luka Gel Ekstrak Pegagan (Centella asiatica (L.) Urban) pada Tikus Putih (Rattus norvegicus)

Abstract

Background: Wounds and hemostasis disorders are conditions that often occur and require effective treatment. Gotu kola (Centella asiatica (L.) Urban) is known to have triterpenoid compounds that can accelerate wound healing and blood clotting. Objective: To test the hemostasis and wound healing activity in gel preparation of ethanol extract of gotu kola herb in white male rats. Methods: The extract was obtained through maceration method and formulated into gel preparations with concentrations of 0%, 1.25%, 2.5%, 3.75%, and 5%. Evaluation included physical quality, stability, and incision wound healing and hemostasis activity testing on male rats. Wounds were treated with the gel twice daily for 14 days, using Bioplacenton® as positive control and gel base as negative control. Results: The ethanol extract of Centella asiatica (L.) Urban can be formulated into a gel preparation that is stable for 2 months. The gel preparation of Centella asiatica (L.) Urban has effectiveness on incision wound healing in rats and stopping bleeding. The most effective concentration is Centella asiatica (L.) Urban ethanol extract gel with 5% concentration which has an average wound healing days of 11.25 ± 0.5 with a wound healing area percentage of 99.75 ± 0.00 days and blood stopping time of 4.015 ± 0.52 minutes. Conclusion: The ethanol extract gel of gotu kola herb is effectively formulated as a topical preparation that is stable and has the potential to accelerate the process of hemostasis and wound healing.