Implikasi Penggunaan Ekstrak Jamur Tiram (Pleurotus ostreatus) Sebagai Antihiperlipid Terhadap Histologi Testis Pada Tikus (Rattus norvegicus)

Abstract

Animal testes with hyperlipidemia will affect the number of Leydig cells and spermatogenic cells, namely spermatogonia, primary spermatocytes and spermatids. Oyster mushroom extract (Pleurotus ostreatus) contains bioactive compounds such as antioxidants, and vitamins such as thiamine, riboflavin, niacin, ascorbic acid, and tocopherol, which contribute to protection from cholesterol. This study aims to determine the effect of oyster mushroom extract on the histology of rat testes with hyperlipidemia. This study used the Completely Randomized Design (CRD) method with 6 treatments and each treatment had 4 replications. The treatment groups of this study consisted of K-, K+, P1, P2, P3 and P4. Each group was given feed and distilled water routinely. The negative control group (K-) was not given any treatment. Positive control group (K+), P1, P2, P3 and P4 were given high-fat feed in the form of used cooking oil and duck egg yolk, then, P1 was given simvastatin while P2, P3 and P4 were given oyster mushroom extract with doses of 150 mg/kgBW, 300 mg/kgBW, 450 mg/kgBW respectively. Testicular histology preparations were made using the paraffin method with Hematoxylin-Eosin (HE) staining. The results showed that oyster mushroom extract (Pleurotus ostreatus) with a dose of 300 mg/kgBW can improve the histological structure of the testes (p<0.05) including the diameter of the seminiferous tubules, the thickness of the germinal epithelium of the seminiferous tubules, Leydig cells, spermatogonium, spermatocytes and spermatids.