Hubungan Polimorfismegen Growth Hormone 1 rs2665802 Terhadap Tinggi Badan dan Kadar Somatotropin Darah pada Perempuan Suku Nias

Abstract

Background: Height is an important indicator of human growth influenced by genetic and hormonal factors. The Growth Hormone 1 (GH1) gene regulates the production of somatotropin, a key growth hormone. The GH1 rs2665802 polymorphism is suspected to contribute to variations in height and somatotropin levels, particularly in specific populations such as Nias women.

Objective: To investigate the association between GH1 rs2665802 gene polymorphism and height as well as somatotropin blood levels in Nias women.

Methods: A cross-sectional study was conducted on 100 Nias women aged 17–24 years. Genotyping was performed using the Nested PCR-RFLP method, and somatotropin levels were measured using ELISA. Data were analyzed descriptively and inferentially using normality tests, Kruskal-Wallis, and Chi-square tests.

Results: The average age was 19.63 ± 1.40 years, height 153.47 ± 4.83 cm, and somatotropin level 739.47 ± 541.28 ng/mL. GH1 rs2665802 genotypes were T/T (49%), T/A (20%), and A/A (31%). Hardy-Weinberg analysis showed disequilibrium (p < 0.0001). No significant difference was found between genotypes and height (p = 0.1452) or somatotropin level (p = 0.8303). However, a significant association was found between genotypes and height categories (p = 0.007), with the A/A genotype more frequently associated with taller stature.

Conclusion: GH1 rs2665802 polymorphism is associated with height category but not significantly with mean height or somatotropin levels. These findings highlight the potential role of genetic variation in determining height phenotype in the Nias female population.