Isolasi Fragmen Gen pi-ta Pada Padi Siporang (Oryza sativa L.) dari Tapanuli Selatan, Sumatera Utara Sebagai Gen Ketahanan Penyakit Blas

Abstract

Siporang rice (Oryza sativa L.) from South Tapanuli Regency, North Sumatra, exhibits resistance to blast disease. This resistance is presumed to be associated with the presence of R genes involved in the plant defense system, one of which is the pi-ta gene. This study aimed to isolate and sequence a fragment of the pi-ta gene from Siporang rice to provide genetic information for plant breeders. The research stages included genomic DNA isolation from Siporang rice, DNA amplification using pi-ta-specific primers, and sequence analysis using NCBI, BLASTn, and MEGA12. Based on the results, the pi-ta gene fragment isolated had a size of 774 bp. BLASTn analysis showed that the gene fragment successfully isolated from Siporang rice (Oryza sativa var. Siporang L.) was confirmed to be the pi-ta gene, with a 99.85% identity to the pi-ta gene sequence from rice cultivar Banks (GQ918477.1). Phylogenetic analysis using the Maximum Likelihood method produced a dendrogram with an evolutionary distance value of 0.16 compared to five other pi-ta gene sequences retrieved from GenBank. The sequence information obtained is expected to be useful for further studies in the development of blast-resistant rice cultivars with optimal yield potential