| dc.description.abstract | Background: Breast cancer remains a major cause of cancer-related mortality in
women. Tinospora crispae (L.) Hook.f. & Thomson, a medicinal plant widely used
in traditional medicine, contains bioactive compounds including alkaloids such as
berberine, which have shown potential anticancer effects.
Objective: This study aimed to evaluate the anticancer activity of berberine-rich
fractions obtained from the stem of Tinospora crispae (L.) Hook.f. & Thomson
against T47D breast cancer cells.
Method: Tinospora crispae compounds was extracted using methanol and was
followed by acid-base fractionation to obtain pH 3 and pH 9 fractions. The presence
and content of berberine were determined using TLC-densitometric analysis.
Cytotoxic activity was evaluated through the MTT assay on T47D cells, and IC₅₀
values were calculated.
Results: TLC-densitometry confirmed the presence of berberine in all samples,
with the pH 9 fraction containing the highest berberine content (2,38 ± 0,04 mg/g),
followed by the pH 3 fraction (2,05 ± 0,08 mg/g), and methanol extract (1,85 ± 0,05
mg/g). Cytotoxicity tests showed that the pH 9 fraction had the strongest cytotoxic
effect with an IC₅₀ of 172.27 ± 8.30 µg/mL, followed by the pH 3 fraction (222.85
± 1.99 µg/mL), and methanol extract (590.92 ± 55.98 µg/mL).
Conclusion: The berberine-rich fraction of Tinospora crispae (L.) Hook.f. &
Thomson stem, especially at pH 9, exhibited promising cytotoxic activity against
T47D breast cancer cells. The results support the potential use of Tinospora crispae
(L.) Hook.f. & Thomson as a natural anticancer agent. | en_US |
