| dc.description.abstract | Background : Gotu kola (Centella asiatica (L.) Urban) is a plant that has benefits
in wound healing with its main content is triterpenoids which include asiaticoside,
centeloside, madecoside and asiatic acid. It was known that polyphenolic compounds
had the potential to enhance platelet aggregation and accelerate coagulation.
Therefore, a comparison was conducted between the Ethanol Extract of Gotu Kola
Herb (EEHP) and the Polyphenol-Rich Extract of Gotu Kola Herb (EKPHP).
Objective : This study aims to determine the difference between Ethanol Extract of
Gotu kola Herb (EEHP) and Polyphenol-Rich Extract of Gotu Kola Herb (EKPHP)
on hemostasis activity.
Methods : The extraction process used the maceration method for the ethanol extract
of gotu kola herb and the polyphenol-rich extract of gotu kola herb, followed by the
fractionation method to obtain the polyphenol-rich extract. Subsequently, hemostasis
activity testing was conducted in vitro by Lee-White method to determine
coagulation time with stopwatch, the Eustrek method to observe hemostasis activity
microscopically, and the platelet aggregation analysis method by a UV-Vis
spectrophotometer.
Results : The normal blood coagulation time was 2.55 ± 0.12. The EDTA + EEHP
(0.5%; 1%; 2%) group had a coagulation time of 120 ± 0,00 (not clotting). The
coagulation times for EEHP 0.5%, EEHP 1%, and EEHP 2% were 1.57 ± 0.06, 1.63
± 0.04, and 1.74 ± 0.07, respectively. Meanwhile, the EKPHP test group showed
better results, with coagulation times of 1.23 ± 0.14 for EKPHP 0.5%, 1.63 ± 0.04
for EKPHP 1%, and 1.74 ± 0.07 for EKPHP 2%. Microscopically, both EEHP and
EKPHP stimulated platelet aggregation and coagulation formation. The percentage
of transmission measured by a UV-Vis spectrophotometer showed that EEHP at 50%
to 80% had transmission values ranging from 46.6% to 63.7%, whereas EKPHP
demonstrated better results, with transmission values ranging from 56.7% to 72% at
50% to 80%.
Conclusion : The polyphenol-rich extract of gotu kola herb effectively accelerated
coagulation time, enhanced hemostasis activity, and increased platelet aggregation
compared to the ethanol extract of gotu kola herb. | en_US |
