| dc.description.abstract | Background: Puguntano (Picria fel-terrae Lour) is a wild plant member of the
genus picria, a member of the Linderniaceae tribe that has the ability to be used as
a source of natural antioxidants. Antioxidants are polar compounds that can help
humans to protect the body from free radical attacks. Bioautography thin layer
chromatography (KLT) test was conducted to identify active compounds that have
activity as antioxidants.
Objective: To determine the antioxidant activity of crude extract and residual
fraction of puguntano leaves (Picria fel-terrae Lour) using thin layer
chromatography (KLT) Bioautography method.
Methods: This research was conducted experimentally which includes the stages
of preparing puguntano leaf samples, phytochemical screening, characterization of
simplisia and extracts, fractionation, and antioxidant tests by thin layer
chromatogaphy (KLT) bioautography.
Results: The simplicia characterization of puguntano leaves obtained water content
of 7,98%, water soluble extract content of 20,8%, ethanol soluble extract content of
9,23%, total ash content of 1,87%, and acid insoluble ash content of 0.3%.
Characterization of the ethanol extract of puguntano leaves obtained water content
of 9,28%, total ash content of 0,13%, and acid insoluble ash content of 0.09%.
Phytochemical screening of simplisia and puguntano leaf extract contains
flavonoids, glycosides, saponins, steroids and tannins. The residual fraction
screening results contain flavonoids, saponins, and tannins. The results of the
antioxidant activity test by KLT Bioautography showed that the residual fraction
using ethyl acetate : methanol (9:1) mobile phase was the most effective as an
antioxidant, with Rf value Rf1 0,61, Rf2 0,75 dan Rf3 0,87
Conclusion: Crude extract and residual fraction of puguntano leaves have
antioxidant activity by KLT Bioautography method. The residual fraction has the
most effective antioxidant activity. | en_US |
