Pengaruh Pemberian Kombinasi Zat Pengatur Tumbuh Indole Acetic Acid (IAA) dan Benzyl Amino Purine (BAP) terhadap Pertumbuhan Tunas Terung Belanda (Solanum Betaceum Cav.) Secara In Vitro

Abstract

NUR ALISA: The Effect of Giving a Combination of Plant Growth Regulators Indole Acetic Acid (IAA) and Benzyl Amino Purine (BAP) on the Growth of Dutch Eggplant Shoots (Solanum betaceum Cav.) In Vitro, supervised by REVANDY ISKANDAR MUDA DAMANIK. Dutch eggplant (Solanum betaceum Cav.) is a high-value horticultural plant with various benefits, but the provision of seeds is still constrained by its susceptibility to pests and diseases. Tissue culture is an alternative solution in the propagation of Dutch eggplant. This study aims to determine the effect of giving a combination of plant growth regulators (PGR) Indole Acetic Acid (IAA) and Benzyl Amino Purine (BAP) on the growth of Dutch eggplant shoots in vitro. This research was conducted at the Plant Biotechnology Laboratory, Faculty of Agriculture, Universitas Sumatera Utara from June to October 2024, this study used a Completely Randomized Design (CRD) with two factors. The first factor is the concentration of IAA (I0 = 0 ppm, I1 (0,5 ppm) = 0.5 ppm, and I2 = 1 ppm), and the second factor is the concentration of BAP (B0 = 0 ppm, B1 = 1 ppm, and B2 = 3 ppm). The results showed that the administration of IAA had no significant effect on the time of shoot emergence, but had a significant effect on the number of leaves and shoots. Similarly, the administration of BAP did not significantly affect the time of shoot emergence, but significantly affected the number of leaves and shoots. The interaction between IAA and BAP did not significantly affect the time of shoot emergence, the number of leaves and shoots. The administration of IAA concentration of 0.5 ppm with BAP 3 ppm gave the best results for the growth of tamarillo in vitro.