Efektivitas Antibakteri Madu Manuka UMF 15+ terhadap Bakteri Pseudomonas Aeruginosa Pada Sekret Penderita Otitis Eksterna Difusa

Abstract

Background: Otitis externa is defined as an inflammatory process due to infection of the cutaneous and subcutaneous ear canal, which can involve the tympanic membrane and pinna. usually appears with symptoms of otalgia, with or without discharge from the ear. Objective: The aim was to analyze the antibacterial effect of Manuka Honey (MM) UMF 15+ on the growth of P.Aeruginosa bacteria in secretions from patients with diffuse OE. Method: This type of research is True Experimental Laboratories with a post-test with control group design model. This research was conducted at Prof. Dr. Chairuddin P. Lubis Hospital. The population and samples used in this study were P. Aeruginosa. The samples used were 24 bacterial cultures from patient ear secretions. The research sample was obtained using the nonprobability sampling method with consecutive techniques. The research was conducted from November 2024 – February 2025. Data analysis was carried out using the ANOVA test if it was normally distributed and if it was not normally distributed using the Kruskal Wallis test. Results: There was a significant difference in the inhibition zone between the negative control and all honey treatments (p <0.001 to 0.003). and indicates that MM has been proven to effectively inhibit growth of resistant P.Aeruginosa bacteria that have become resistant to Ampicillin/Sulbactam, Aztreonam, Ceftazidime, Ceftriaxon, Cefepime and Cotrimoxazole. Conclusion: Manuca Honey UMF 15+ at all concentrations significantly inhibits bacterial growth compared to the negative control (DMSO 1%) and indicates that MM is proven effective in inhibiting the growth of resistant P. Aeruginosa.