Efektivitas Antijamur Ekstrak Daun Jeruk Nipis (Citrus aurantifolia S) terhadap Candida albicans pada Kandidiasis Lidah (In Vitro)

Abstract

Introduction Candidiasis is the most common fungal infection of the oral cavity and is caused by the overgrowth of commensal Candida species. Treatment of candidiasis usually uses synthetic drugs but often causes side effects such as resistance, expensive, difficult usage rules, nausea, headaches and stomach aches. Indonesia has many herbal plants such as lime leaves. Lime leaves can be used as an antifungal because they contain alkaloids, saponins, flavonoids, polyphenols which function to inhibit growth and disrupt cell stability, causing fungal lysis. Purpose of the study to determine the inhibitory power and differences, Minimum Inhibitory Rate, Minimum Bactericidal Rate, and number of colonies of lime leaf extract at concentrations of 15%, 30%, 45% and 60% against Candida albicans. Method is experimental laboratory posttest control group design with 4 repetitions. Extract preparation using maceration method. Inhibitory power testing using diffusion method, determination of MIC and MBC using dilution method. Calculation of the number of colonies using Colony counter. Parametric analysis with One way Anova Test and Post-hoc LSD Test with significance value p <0.05, if the data obtained is not normally distributed and homogeneous, non-parametric analysis is carried out with Kruskal-Wallis Test and Mann-Whitney Test. Results of the research on the inhibitory power of lime leaf extract at concentrations of 15%, 30%, 45% and 60% diameter 11.31 ± 0.24, 16.31 ± 0.24, 20.31 ± 0.38, 30.00 ± 0.41. Minimum Inhibitory Concentration at 15% and Minimum Killing Concentration at 60%. The results of the calculation of the number of colonies at concentrations of 15%, 30%, 45% and 60% were 149.75±18.76 CFU/ml, 41.00±7.70 CFU/ml, 6.75±2.06 CFU/ml and 0 CFU/ml. Conclusion lime leaf extract is effective in reducing the growth of Candida albicans fungus.