Pengaruh Pemberian DMSO (Dimetil sulfoksida) dengan Larutan Madu Terhadap Kualitas Sperma Ikan Tawes (Barbonymus gonionotus) pada Pasca Kriopreservasi

Abstract

Tawes (Barbonymus gonionotus) is a type of freshwater fish originating from Indonesia and is known as a plant eater, over time this fish is increasingly cultivated by the community. The success of fish farming is highly dependent on hatchery technology, especially in the preservation of fish spema (cryopreservation). The purpose of this study was to determine the effect of honey addition on motility and viability of tawes spermatozoa (Barbonymus gonionotus) in post-cryopreservation. This research was conducted in March-April 2025. The method used in this study was a completely randomized design (CRD) with 5 treatments and 3 replicates. The samples used were 3 male broodstock, with an average male fish weight of 22.53 ± 0.86 grams and length of 118 ± 4.04 cm, sperm pH around 7, thick and milky white color. In testing samples of tawes sperm cryopreservation (Barbonymus gonionotus) with Treatment using Dimethyl sulfoxide (DMSO) cryoprotectant with honey solution. The treatments are (P0) Honey solution without DMSO, (P1) Honey solution with 5% concentration of DMSO, (P2) Honey solution with 5% concentration of DMSO, (P2) Honey solution with 5% concentration of DMSO, (P1) Honey solution with 5% concentration of DMSO, (P2) Honey solution with 5% concentration of DMSO