| dc.description.abstract | Background: Treatment of acne with antibiotics takes a long time, thus risking the emergence of bacteria resistance to antibiotics. Moringa seed oil can be used as an alternative because it is rich in fatty acids such as oleic acid and palmitic acid which have potential as antibacterials. So, in this study moringa seed oil was formulated into a water-based serum gel preparation that is more suitable for acne therapy, especially on oily skin, in addition to the good spreading power of the gel preparation so that it can provide more effective absorption of active substances.
Objectives: This study aimed to formulate a serum gel preparation of moringa seed oilt that meets the physical evaluation requirements for serum gel preparations and analyze the effect of moringa seed oil concentration on the antibacterial activity of Propionibacterium acnes.
Methods: In the initial stage, moringa seed oil was tested for antibacterial activity to determine the minimum inhibitory concentration (MIC) of the oil. Furthermore, serum gel preparations were made with various concentrations, namely 0 (F0), 1 (F1), 2 (F2), and 3% which were then followed by preparation evaluation. Physical evaluation of serum gel preparation includes organoleptic test, homogeneity, spreadability, pH, viscosity, cycling test, and stability at room temperature for 12 weeks. Furthermore, testing the antibacterial activity of moringa seed oil preparations was carried out against Propionibacterium acnes using the well diffusion method.
Results: The minimum inhibitory concentration of moringa seed oil was at a concentration of 0.25% with an inhibitory diameter of 10.67 ± 0.59 mm. The white transparent serum gel preparation has a distinctive aroma of lavender flowers and is homogeneous. The pH values obtained were 5.12 - 5.90, viscosity 1986.33 cps - 2415.00 cps, and spreadability 5 - 6.6 cm. The serum gel preparation was stable for 12 weeks of storage and cyling test. The results of the antibacterial test of moringa seed oil (Moringa oleifera L.) serum gel preparation against Propionibacterium acnes showed that in F0 (blank), the inhibition zone (medium) was obtained, which was 8.33 ± 0.015 mm. While the inhibition zones of F1 (1%), F2 (2%), F3 (3%) are 16.70 ± 0.10 mm, 17.40 ± 0.10 mm, and 18.30 ± 0.10 mm, respectively, which are included in the inhibition (strong).
Conclusions: Moringa oleifera seed oil can be formulated into a serum gel preparation that was stable during 12 weeks storage at room temperature and met the physical requirements of the preparation. Gel preparation with 3% concentration showed the highest antibacterial activity against Propionibacterium acnes. | en_US |
