Dose Response Populasi Lulangan (Eleusine Indica) Resisten Ganda-Glifosat dan -Glufosinat terhadap Glifosat, Glufosinat Dan Paraquat

Abstract

This study aimed to evaluate the dose response of goosegrass (Eleusine indica) populations suspected of having developed resistance to the herbicides glyphosate and glufosinate. The resistant population (EKM) was collected from PTPN II Kwala Madu Plantation, while the susceptible population (EPB) was obtained from Padang Bulan Ward, an area with no history of herbicide exposure. The experiment was arranged in a Randomized Block Design (RBD) with four replications. The number of herbicide dose levels varied by treatment: six doses for glyphosate (tested on both EKM and EPB) and for glufosinate (on EPB), five doses for glufosinate (on EKM), and eight doses for paraquat (on both populations). Observed parameters included mortality, dry weight, and visual symptoms of injury. The results showed that the EKM population exhibited high Resistance Index (RI) values based on LD₅₀: glyphosate (>34,560 g a.i./ha), glufosinate (12.72), and paraquat (8.70). Based on GR₅₀, EKM also demonstrated significant resistance: glyphosate (4.80), glufosinate (6.79), and paraquat (5.20). Compared to EPB, EKM showed lower mortality, higher dry weight, and milder injury symptoms across all tested doses. These findings confirm that the EKM population has developed multiple resistance to glyphosate and glufosinate, as well as moderate tolerance to paraquat. The results highlight the urgent need to implement integrated weed management strategies, including herbicide mode-of- action rotation, to prevent further spread of herbicide resistance.