Isolasi dan Identifikasi Golongan Senyawa Flavonoida dari Kulit Batang Pirdot (Saurauia vulcani Korth.)

Abstract

Isolation and identification of flavonoid compound from the bark of Pirdot (Saurauia vulcani Korth.) had been carried out through the maceration extraction stage with methanol solvent. The methanol extract dissolved with ethyl acetate was separated and concentrated. The concentrated extract of ethyl acetate was dissolved with methanol and partition extracted with n-hexane. The methanol layer is then evaporated until all the methanol has evaporated. The concentrated methanol extract was then analyzed by TLC, then separated by column chromatography with silica gel as the stationary phase and chloroform: ethyl acetate (90:10, 80:20, 70:30, 60:40, 50:50, 40:60, 30:70, 20:80, and 10:90) v/v as the mobile phase. The second fraction with a value of Rf = 0.55 uses chloro: ethyl acetate (30:70) v/v. Based on the results analyzed with a UV-Visible Spectrophotometer, it shows that there are two absorption bands with a wavelength (λmax) of 311 nm in band I and 272 nm in band II. The results of FT-IR spectrum analysis show the presence of O-H, CH sp3 , C=O, C=C aromatic, C-C-O, CH sp2 groups. The results of 1H-NMR spectrum analysis show the of H-3 protons at δ = 6.7336 (ppm). Infrared (FT-IR), and Proton Nuclear Magnetic Resonance Spectrophotometry (¹H- NMR). Spectroscopic data shows that the isolated compounds are flavones.