Kinerja Nikel/Grafena Berlapis Nano dan Perak/Grafena Berlapis Nano dari Cangkang Kemiri Menggunakan Metode Pirolisis Sebagai Antibakteri Staphylococcus aureus

Abstract

Research on the Performance of Nickel/Graphene Nano Sheets (Ni/GNS) and Silver/Graphene Nano Sheets (Ag/GNS) from Candlenut Shells Using Pyrolysis Method as Antibacterial Staphyloccocus aureus has been conducted. The purpose of this study was to analyze the effect of Ni/GNS and Ag/GNS concentrations in achieving optimum performance on the antibacterial activity of Staphylococcus aureus, with a concentration variation of Ni/GNS and Ag/GNS of 1%, 5%, 10%, 20%, and 30%. This research is a laboratory experimental and descriptive research. Graphene Nano Sheets was synthesized using pyrolysis method, synthesis of Ni/GNS and Ag/GNS of each variation was done by blending method. Analysis of GNS, Ni/GNS, and Ag/GNS in each variation was carried out using Gel Diffusion Assay, Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC), XRD, SEM-EDX and Multimeter, respectively. The inhibition zone showed that Ni 30%/GNS was the largest (28.69 mm) followed by Ag 10%/GNS (17.53 mm) and GNS (10.76 mm). The MIC and MBC results show that the presence of GNS and the type of metal affects the growth of bacteria, where negative growth of Staphylococcus aureus bacteria for Ag 1-30%/GNS, while GNS and Ni 1-30%/GBN have positive growth of Staphylococcus aureus bacteria. The presence of peaks at 2θ = 44.5°, 31°, and 23.5° (XRD data) indicates that Ni and Ag have been deposited into GNS and GNS was successfully synthesized. The presence and abundance of Ag and Ni in the GNS were consistent with SEM-EDX. The analysis of Conductivity (DHL), Power Density and Energy Density of each GNS, Ni/GNS and Ag/GNS also showed that GNS, metal type and metal concentration can affect the activity of the alloy. The data showed that GNS, variation of metal concentration and type can affect the antibacterial performance of the alloys.