Mikropropagasi Tunas Manggis (Garcinia mangostana L.) Menggunakan Eksplan Shoot Tip dengan Penambahan NAA dan BAP

Abstract

Mangosteen is a fruit plant that contains many properties, especially found in mangosteen skin. The generative propagation of mangosteen by utilizing seeds is less profitable because mangosteen seeds are recalcitrant. Conventional vegetative propagation has not been able to fulfill the needs of mangosteen seedlings properly. Therefore, vegetative propagation using tissue culture method is needed to meet the needs of mangosteen seedlings. This study aims to determine the effect of the addition of NAA and BAP on the growth of mangosteen (Garcinia mangostana L.) shoot tips in vitro. This research was conducted at the Biotechnology Laboratory, Faculty of Agriculture, University of Sumatera Utara, Medan City, North Sumatra Province from January to May 2025. This study used the RAL method (Completely Randomized Design) with two factors. The first factor is the addition of NAA with concentrations: 0 ppm, 1 ppm, and 2 ppm. The second factor is the addition of BAP with concentrations: 0 ppm, 2 ppm, 4 ppm, and 6 ppm. The results showed that the addition of NAA had a significant effect on the parameters of apical bud height, while the addition of BAP had no significant effect on all parameters. The interaction of the addition of NAA and BAP had a significant effect on the total number of buds and the age of bud emergence, while the effect was not significant on all other observed parameters.