Penentuan Kadar β-Karoten dalam CPO (Crude Palm Oil) Menggunakan Spektrofotometri UV-Visible di Pusat Penelitian Kelapa Sawit

Abstract

Beta-carotene is a natural compound found in crude palm oil (CPO) and gives the oil its red color. CPO contains relatively high levels of beta-carotene, making it a source of provitamin A and a natural antioxidant.. This study aimed to determine the βcarotene content in CPO using the UV-Vis Spectrophotometry method in accordance with the MPOB quality standards. Using UV-VIS Spectrophotometry at the Palm Oil Research Center. The UV-Visible Spectrophotometry method is an analytical method that uses UV and visible wavelengths as absorption areas to detect compounds. The results of the β-carotene content determination used an absorbance of 446 nm. Based on the research results, the β-carotene value in the CPO sample code D was 705.48 ppm, code E 498.88 ppm, and code F 309.75 ppm. meets the MPOB quality standard of 500-700 ppm. This study successfully measured the β-carotene content in crude palm oil (CPO) using UV-Vis spectrophotometry at a wavelength of 446 nm. The analysis results showed that the CPO samples coded D (705.48 ppm) and E (498.88 ppm) had β-carotene content that nearly met or was within the MPOB quality standard range (500–700 ppm), while the sample coded F (309.75 ppm) was below the standard limit. Therefore, it can be concluded that several samples met the β-carotene quality standard as stipulated by the MPOB, and the use of the UV-Vis spectrophotometry method proved effective for this analysis.