| dc.description.abstract | Black cumin (Nigella sativa L.) is an herbal plant that has various
pharmacological and culinary benefits. The production of black cumin in Indonesia
is still low, so Indonesia imports black cumin seeds from India, Egypt, Syria, and
South Africa to meet the high demand for black cumin seeds. This study aims to
determine the effect of IAA and BAP concentration and their interaction on MS
basal medium on the in vitro growth of black cumin (Nigella sativa L.) callus. This
research was conducted at the Plant Biotechnology Laboratory, Faculty of
Agriculture, University of North Sumatra, from December 2024 to May 2025. This
research uses a Completely Randomized Design (CRD) with two factors. The first
factor is the concentration of Indole Acetic Acid (IAA): I0 (0 ppm), I1 (1.75 ppm),
I2 (2.0 ppm), and I3 (2.25 ppm). The second factor is Benzyl Amino Purine (BAP):
B0 (0 ppm), B1 (1.25 ppm), B2 (1.5 ppm), and B3 (1.75 ppm). The parameters
observed in this study were the percentage of living explants (%), the percentage of
explants forming callus (%), the percentage of explants forming shoots (%), days
to callus appearance (d), fresh weight of callus (g), callus color, and callus texture.
The research results showed that the application of IAA and BAP significantly
affected the parameters of explants forming callus, days to callus appearance, and
fresh weight of callus, with the best treatment being I1B3 (1.75 ppm IAA + 1.75
ppm BAP), which induced the fastest callus appearance time of 29.50 days, the
highest fresh weight of callus at 0.0404 grams, green color, and compact callus
texture. | en_US |
