Isolasi dan Identifikasi Golongan Senyawa Flavonoida dari Daun Iriancis (Impatiens hawkeri W.Bull)

Abstract

Isolation and identification of flavonoid compounds from the leaves of the Iriancis (Impatiens hawkeri W.Bull) have been carried out through the maceration extraction stage with methanol solvent. Concentrated methanol extract was dissolved with ethyl acetate. Concentrated ethyl acetate extract was dissolved with methanol and partitioned using n-hexane. The methanol layer was then evaporated until all the methanol had evaporated. The concentrated methanol extract was then analyzed by TLC, then separated using coloumn chromatography with silica gel as the stationary phase and chloroform : methanol (90:10, 80:20, 70:30, 60:40) v /v as the mobile phase. The pure compound obtained was in the form of a yellow armorphous solid with a mass of 5.3 mg with an Rf value 0f 0,28 using n-hexane : ethyl acetate eluent 70 : 30 v /v. Based on UV-Vis spectroscopy analysis with methanol solvent shows a wavelength (λmax) of 280 nm and 355 nm. The FT-IR spectrum shows the presence of O-H, C-H sp3 , C=O ketone, C=C aromatic, C-H sp3 bend, C-O-C, and C-H sp2 groups respectively at wave numbers 3427,51 cm-1 ; 2922,16 cm-1 ; 1734,01 cm-1 ; 1463,97 cm1 ; 1381,03 cm-1 ; 1122,57 cm-1 ; 721,38 cm-1 . The 1H-NMR spectrum shows the presence of protons H-2’, H-6’, H-3’, H-5’, H-8, H-6, OCH3. Based on the data obtained, it shows that the isolated compound is a flavonoid compound of the flavonol group.