| dc.description.abstract | Background: Periodontal disease can cause significant alveolar bone damage and in severe cases can lead to tooth loss. Periodontal therapy aims to regenerate all tissues damaged by periodontal disease, one of which is bone regeneration. At this time, regenerative approaches such as bone grafting have been developed. In recent years, with the innovation of bone tissue engineering, biomaterials have focused on the design of scaffolds that are physicochemically suitable for cell attachment, proliferation, migration, differentiation, and formation of specific organ tissues. The main goal of scaffold design is to mimic the original extracellular matrix (ECM) of the target network as much as possible. Since no ideal biomaterial can achieve all the properties required for a wide range of tissue engineering applications, composite scaffolding is a better choice. Composite scaffolding has two or more biomaterials combined to improve its mechanical properties or functional efficiency or both. Biomimetic materials have the potential to mimic many of the roles of ECM in tissues, where we get these materials from nature such as chitosan oligosaccharides (COS), hyaluronic acid (HA), and collagen. Objective: To analyze the effectiveness of the combination of chitosan oligosaccharides, hyaluronic acid, and collagen as scaffolds on osteoblast proliferation and migration. Methods: Laboratory experimental research in vitro with post-test control group design of 4 sample groups, namely alloplast GamaCHA, COS scaffold, COS-HA scaffold, and COS-HA-Kol scaffold. Proliferation and migration tests were performed on osteoblast cells. The data were analyzed by the One-Way ANOVA test, Dunnet's post-hoc test, and LSD post-hoc test. Results: The results showed that there were significant differences in osteoblast proliferation on days 1, 3, and 7 and osteoblast cell migration at 24 hours and 48 hours between the GamaCHA alloplast, COS scaffold, COS-HA scaffold combination, and COS-HA-Kol scaffold combination. Conclusion: The combination of chitosan oligosaccharides, hyaluronic acid, and collagen as scaffolds has an effect on osteoblast proliferation and migration. | en_US |
