Uji Aktivitas Penghambatan Enzim Tirosinase Dari Ekstrak Etanol Daun Pirdot (Saurauia vulcani Korth.)

Abstract

Background: Materials used to overcome hyperpigmentation often caused side effects. Therefore, natural materials were needed to address hyperpigmentation while minimizing those side effects. The pirdot plant (Saurauia vulcani Korth) was a typical plant from North Sumatra known for its traditional medicinal uses. However, research on the tyrosinase enzyme inhibitory activity of this plant was still limited. Objective: The aim of this research was to evaluate the total phenolic content, total flavonoid content, antioxidant activity, and tyrosinase enzyme inhibitory activity of the ethanol extract of pirdot leaves (S. vulcani). Method: The total phenolic content was determined using the Folin-Ciocalteu reagent, while the total flavonoid content was measured using the AlCl₃ reagent. Antioxidant activity was tested using the DPPH method, and the tyrosinase enzyme inhibitory activity was also evaluated. Results: The results of the study indicated that the ethanol extract of pirdot leaves (S. vulcani) contained 138.277 mg GAE/g extract of total phenolic compounds and 40.75 mg QE/g extract of total flavonoid compounds. The IC₅₀ value for antioxidant activity was 42.33 μg/mL, while the IC₅₀ value for tyrosinase enzyme inhibition was 1330.14 μg/mL. Conclusion: The results of the study concluded that the ethanol extract of pirdot leaves (S. vulcani) has phenol and flavonoid levels. The antioxidant activity of pirdot leaves destroys strongly and the inhibitory activity of the tyrosinase enzyme destroys very weakly.