Uji Efektivitas Ekstrak Gelatin Tulang Ikan Kembung (Rastrelliger sp) Terhadap Jumlah Sel Osteoklas Pada Penyembuhan Tulang Mandibula Tikus Galur Wistar

Abstract

Bone defects are bone damage in the maxillofacial area that requires therapy using a graft material, namely xenograft in the form of a scaffold. Gelatin is used as a potential scaffold due to the lack of body immune response and high biocompatibility. Fish bones have become one of the sources of halal gelatin. The use of mackerel bone (Rastrelliger sp) as a source of gelatin can be used as a scaffold for treating bone defects. This study aimed to examine the effectiveness of the gelatin extract of mackerel (Rastrelliger sp) on the number of osteoclast cells in the healing of mandibular bone of male Wistar rats. This study is an in vivo experimental study with 24 Wistar rats (Rattus novergicus) as the research sample and divided into 6 groups. Three treatments with observations every 7th day and 14th day. Defect made on the left lateral mandibular bone with a size of 5x5 mm. The specimens were then stained with Hematoxylin Eosin (HE) staining to observe changes in osteoclast cells that occurred on the 7th and 14th days. The results showed the effectiveness of mackerel bone gelatin extract (Rastrelliger sp) on decreasing the number of osteoclast cells in healing mandibular bone defects of Wistar rats. The negative treatment group that was given 0.5 mg gelatin showed a better reduction in the number of osteoclasts compared to the control group without treatment.