Isolasi dan Identifikasi Golongan Senyawa Flavonoida dari Kulit Batang Tumbuhan Ceremai (Phyllanthus acidus (L.) Skeels)

Abstract

The cherry plant (Phyllanthus acidus (L.) Skeels) is one of the herbs that has the potential as a medicine. One of the bioactive compounds that plays a role in this is flavonoids. Isolation and identification of flavonoid compound groups from the bark of the cherry plant (Phyllanthus acidus (L.) Skeels) has been carried out, starting with the maceration extraction stage with methanol solvent. The concentrated methanol extract was repeatedly extracted with ethyl acetate until negative with the 5% FeCl3 test. The ethyl acetate extract was dissolved with methanol and partitioned with n- hexane until clear. The concentrated methanol extract was then analyzed by TLC, then separated by column chromatography with silica gel as the stationary phase with ethyl acetate chloroform eluent (90:10, 80:20, 70:30, 60:40, 50:50, 40:60, 30:70, 20:80, and 10:90) as the mobile phase. The eighth fraction was selected for purification by column chromatography with silica gel as the stationary phase with chloroform: ethyl acetate (40:60) as the mobile phase. The pure compound obtained was in the form of a yellow amorphous solid as much as 7 mg with an Rf value = 0.52 using chloroform: ethyl acetate (20:80) %. Furthermore, the obtained compound was analyzed by UV- Visible Spectrophotometer, Infrared Spectrophotometer (FT-IR), and Proton Nuclear Magnetic Resonance Spectrophotometer (1H-NMR). Spectroscopic data shows that the isolated compound is an isoflavone.