Pengaruh Zat Pengatur Tumbuh GA3 dan Kinetin terhadap Pertumbuhan Biji Jeruk Siam Madu (Citrus nobilis L.) Asal Siboras Simalungun secara In Vitro

Abstract

This study aims to determine the effect of giving a combination of GA3 and Kinetin in vitro on the seeds of Siam Madu Orange (Citrus nobilis L.) from Siboras Simalungun. This research was conducted at the Biotechnology Laboratory, Faculty of Agriculture, Universitas Sumatera Utara, Medan with an altitude of ± 25 meters above sea level from December 2024 to March 2025. This study used a Completely Randomized Design (CRD) method with 2 treatment factors and 3 replications. The first factor is the concentration of GA3 (G), namely G0 (0 ppm), G1 (2 ppm), and G2 (4 ppm). The second factor is the concentration of Kinetin (K), namely K0 (0 ppm), K1 (2 ppm), and K2 (3 ppm). The parameters observed in this study included the percentage of live explants, days to shoot emergence, number of shoots, shoot height, number of roots, root length, number of leaves, and stem diameter. The results showed that the application of GA3 had a significant effect on the day of shoot emergence, shoot height, and number of roots, but had no significant effect on the number of shoots, root length, number of leaves, and stem diameter. The application of Kinetin had a significant effect on shoot height and root length, but had no significant effect on days to shoot, number of shoots, number of roots, number of leaves, and stem diameter, while the interaction between the treatment combination of GA3 and Kinetin had a significant effect on days to shoot, but had no significant effect on the number of shoots, shoot height, number of roots, root length, number of leaves, and stem diameter.