Perbandingan Aktivitas Antibakteri Ekstrak Mae dan Maserasi dari Daun Binahong (Anredera cordifolia (Ten.) Steenis) terhadap Propionibacterium acnes dan Staphylococcus aureus

Abstract

Background: Binahong leaves are one of the typical Indonesian plants that have wound healing, analgesic, antioxidant, antimicrobial, antidiabetic, antihyperlipidemia, antihypertensive and anticancer properties. It contains flavonoid, tannin, alkaloid, and saponin compounds that have antibacterial activity. Objective: To determine the antibacterial activity of ethanol extract of binahong leaves against Propionibacterium acnes and Staphylococcus aureus and to see the difference in antibacterial activity of ethanol extract of binahong leaves extracted by MAE and maceration methods. Methods: This study includes the preparation of simplisia, characterization of simplisia, phytochemical screening, extraction by MAE and maceration methods using 70% ethanol and testing the antibacterial activity of ethanol extracts of binahong leaves diluted using DMSO into several concentrations, namely 500 mg/ml; 200 mg/mL; 100 mg/mL; 50 mg/mL; 25 mg/mL; 12,5 mg/mL; 6,25 mg/mL; 3,125 mg/mL; 1,56 mg/mL; 0,78 mg/mL with positive control of Chloramphenicol against Propionibacterium acnes and Staphylococcus aureus by agar diffusion method. Results: Ethanol extract of binahong leaves extracted by MAE and maceration had antibacterial activity against Propionibacterium acnes with KHM at concentrations of 6,25 mg/mL; 3, 125 mg/mL at 7,48±0,17 mm, respectively: 7,53±0,08 mm and against Staphylococcus aureus with KHM at concentration 1,56 mg/mL of 6,55±0,06 mm and 7,2±0,7 mm respectively. Conclusion: Ethanol extract of binahong leaves extracted by MAE and maceration methods have antibacterial activity against Propionibacterium acnes and Staphylococcus aureus bacteria and there is no significant difference between the two methods.