Formulasi Nanogel Quersetin dan Evaluasi Stabilitas Fisik, Aktivitas Anti-Inflamasi pada Tikus yang Diinduksi oleh Karagenan

Abstract

Inflammation is a tissue response to damaging physical or chemical stimuli. This stimulus causes the release of inflammatory mediators, which cause an inflammatory reaction in the form of heat, pain, redness, swelling, and impaired function. One active substance that can inhibit neutrophil infiltration and reduce plasma levels of inflammatory cytokines is quercetin. Quercetin also functions as an antioxidant. In this study, quercetin was made into a nanogel preparation. The advantage of nanogel is that it penetrates the skin more easily, thus improving its anti-inflammatory effectiveness. To determine the concentration of quercetin that can be formulated into nanogel using carbopol 940 as a gel-forming agent and to test whether it meets the characteristics of the gel and to test the anti-inflammatory effectiveness on carrageenan-induced rat paws. Quercetin with concentrations of 0.06%, 0.08%, and 0.1% was formulated into nanogel preparations. Anti-inflammatory activity was tested in mice injected with 1% carrageenan to determine the effective topical dose. The initial stage of the formulation was the preparation of nanosuspensions with quercetin concentrations of 0.06g (F1), 0.08g (F2), 0.1g (F3). The nanosuspensions were added to the gel base with stirring using a homogenizer to obtain nanogels. The nanogels were evaluated for physical stability by storage at various temperatures for 12 weeks and cycling tests. After that, their anti-inflammatory properties were tested in mice compared to voltaren emulgel. Quercetin nanogel was stable during 12 weeks of storage from organoleptic, homogeneity, pH (4.5-6.0), viscosity (7031-9894 m.Pa.s), spreadability (4.5-7.1 cm2), particle size (40-70nm). The anti-inflammatory effect of F3 nanogel showed an anti-inflammatory inhibitor of 80.87±3.70%. Based on the results of this study, quercetin nanogel which has more effective anti-inflammatory activity in mice is at a quercetin concentration of 0.1% (F3) in mice compared to F1 and F2.