Uji Efektivitas Nanofungisida Berbasis Perak Nitrat dan Trichoderma virens dalam Menekan Pertumbuhan Ganoderma Boninense Secara In Vitro

Abstract

Basal stem rot disease in oil palm plantations caused by the pathogenic fungus Ganoderma boninense remains a serious problem and causes economic losses. Studies on the effect of silver nanoparticles (AgNPs) on controlling G. boninense have not been widely conducted. The commonly used methods for synthesizing silver nanoparticles are physical and chemical methods, but these methods have adverse effects on the environment and living organisms, so other safer and more effective methods are needed. This study aims to test the potential of silver nanoparticles (AgNPs) synthesized with Trichoderma virens (Tv) in suppressing the growth of G. boninense in vitro. This study used a Complete Randomized Factorial Design with two treatment factors, namely: 1) application methods consisting of poisoning and dual culture, and 2) comparison of T. virens with AgNP with control treatment, D'Flava, a fungicide with the active ingredient Mancozeb, Tv 1:4 AgNP, Tv 1:5 AgNP, Tv 1:10 AgNP, Tv 1:20 AgNP. The results showed that the inhibition test of G. boninense using the poisoning method was more effective than the dual culture method, with the best treatment being Tv 1:10 AgNP, yielding an average growth of 30.87 mm at 14 days after inoculation (DAI). The application of AgNPs caused abnormal growth of G. boninense hyphae, namely coiled, curled, twisted, broken, and lysed hyphae.